LOCUS       pLV-[hsa-miR-187]        9163 bp    DNA     circular     20-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7084..7944
                     /label=Amp(R)
     promoter        8145..8685
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5689..5923
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6498..6953)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5995..6125
                     /label=SV40 pA
     misc_feature    6126..6339
                     /label=SV40 ori
     misc_feature    5010..5606
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3628
                     /label=intron
     CDS             3675..5000
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3418
                     /label=hsa-mir-187 precursor
BASE COUNT     2157 a      2391 c      2514 g      2101 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct gttcttttac 
     3121 agcttcctca tcgggatgca cagcaagtcg gattccccaa gcagaagctg ggcagcccac 
     3181 ctggaagagg aggccaggca ggtgctgcag gtcgggctca ccatgacaca gtgtgagacc 
     3241 tcgggctaca acacaggacc cgggcgctgc tctgacccct cgtgtcttgt gttgcagccg 
     3301 gagggacgca ggtccgcagc agagcctgct ccgcttgtcc tgagggactc gacacagggg 
     3361 actgcacaga gaccatggga aagtccaggc tctggtgggc aggtgggtgg ggtgttgggc 
     3421 tagctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga 
     3481 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt 
     3541 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt 
     3601 ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa ctacccctct agagtcgagc 
     3661 taccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt 
     3721 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg 
     3781 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg 
     3841 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg 
     3901 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct 
     3961 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact 
     4021 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct 
     4081 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt 
     4141 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg 
     4201 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg 
     4261 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg 
     4321 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca 
     4381 aggacccacc ggtcgccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg 
     4441 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc 
     4501 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc 
     4561 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg 
     4621 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc 
     4681 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg 
     4741 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc 
     4801 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg 
     4861 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc 
     4921 tcggcttcac cgtcaccgcc gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga 
     4981 cccgcaagcc cggtgcctga gcggccgcgt cgacaatcaa cctctggatt acaaaatttg 
     5041 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 
     5101 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 
     5161 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 
     5221 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 
     5281 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 
     5341 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 
     5401 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 
     5461 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 
     5521 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 
     5581 ctccctttgg gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact 
     5641 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 
     5701 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac 
     5761 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 
     5821 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 
     5881 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 
     5941 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 
     6001 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 
     6061 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 
     6121 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 
     6181 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 
     6241 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt 
     6301 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg 
     6361 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 
     6421 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 
     6481 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 
     6541 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 
     6601 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 
     6661 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 
     6721 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 
     6781 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 
     6841 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 
     6901 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc 
     6961 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 
     7021 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 
     7081 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 
     7141 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 
     7201 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 
     7261 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 
     7321 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 
     7381 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 
     7441 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 
     7501 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 
     7561 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 
     7621 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 
     7681 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 
     7741 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 
     7801 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 
     7861 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 
     7921 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 
     7981 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 
     8041 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
     8101 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     8161 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     8221 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     8281 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     8341 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     8401 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     8461 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     8521 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     8581 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     8641 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     8701 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     8761 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     8821 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     8881 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     8941 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 
     9001 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 
     9061 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag 
     9121 cgcgcaatta accctcacta aagggaacaa aagctggagc tgc 
//
